中国人兽共患病学报2011，Vol.27Issue(9)：778-782,5.
乙型脑炎病毒NS5蛋白原核表达与亚细胞定位研究
Prokaryotic expression and subcellular localization of Japanese encephalitis virus NS5 protein
栗永茂 1曹胜波 2陈龙 2霍雨艳 1熊涛 1吴光旭1
作者信息
- 1. 长江大学生命科学学院,荆州 434025
- 2. 华中农业大学动物科技学院动物医学院,武汉430070
- 折叠
摘要
Abstract
The NS5 gene of Japanese encephalitis virus was amplified by PCR and cloned into the prokaryotic expression vector pET-28a. The recombinant NS5 protein was expressed highly under induction of IPTG in the E. Coli. Using BALB/c female mice immuned with the purified recombinant NS5 protein, NS5 protein-specific polyclonal antibody were prepared. Meanwhile, BHK-21 cells were transfected with eukaryotic expression plasmid pcDNA-NS5-EGFP of NS5 gene and green fluorescent protein (EGFP) fusion expression. Finally, the subcellular localization of NS5 protein in BHK-21 cells was observed by using laser confocal fluorescence microscope. The result indicates that the protein particles are irregularly distributed in the whole cell and particularly in the cytoplasm.关键词
NS5/原核表达/亚细胞定位/乙型脑炎病毒Key words
NS5/ prokaryotic expression/ subcellular localization/ Japanese encephalitis virus分类
医药卫生引用本文复制引用
栗永茂,曹胜波,陈龙,霍雨艳,熊涛,吴光旭..乙型脑炎病毒NS5蛋白原核表达与亚细胞定位研究[J].中国人兽共患病学报,2011,27(9):778-782,5.
