中国药理学通报2011，Vol.27Issue(11)：1504-1509,6.DOI:10.3969/j.issn.1001-1978.2011.11.008
Caco-2细胞对负载维生素D3的纳米粒的摄入研究
The uptake of vitamin D3 imbed by self-assembled sodium alginate nanoparticles with caco-2 cells
摘要
Abstract
Aim To investigate the uptake of vitamin D3 imbed by self-assembled sodium alginate nanoparti-cles labeled by FITC ( sSAN-VD3-FITC ), and the influential factors with Caco-2 cells. And to study the transmembrane transport of it with Caco-2 cell absorption model. Methods Laser scanning confocal microscope ( LSCM ) was utilized to exam the uptake of sSAN-VD3-FITC absorbed by Caco-2 cells. The effect of drug incubation time on the uptake was analyzed. Caco-2 cell absorption model was used to study the transmembrane transport of sSAN-VD3-FITC. The apparent permeability coefficient ( Papp ) was calculated and the fluorescence intensity was detected to quantitatively assay the influence of drug incubation time on the transmembrane transport. Results It took only lh for the appearance of sSAN-VD3-FITC fluorescenceparticle diameter in Caco-2 cells. It was brighter when incubated for 4 h. The fluorescence intensity and the accum transport quantity of sSAN-VD3-FITC group were higher than those of the control group ( P < 0. 01 ), and they were higher while the time was longer ( P < 0. 05 ). Papp was fairly stabilized, but it was smaller while the time was longer. Conclusions sSAN-VD3 can be uptaken by Caco-2 cells, and can also do transmembrane transport with Caco-2 cell absorption model in a time-dependent manner. It indicates that sSAN-VD3 can be absorbed into blood via gastrointestinal tract pathway.关键词
Caco-2细胞/自组装海藻酸钠纳米粒/维生素D3/FITC/摄取/跨膜转运Key words
Caco-2 cell/ self-assembled sodium alginate nanoparticles/ vitamin D3/ FITC/ uptake/ transmembrane transport分类
医药卫生引用本文复制引用
高艳丽,刘赛..Caco-2细胞对负载维生素D3的纳米粒的摄入研究[J].中国药理学通报,2011,27(11):1504-1509,6.基金项目
国家高技术研究发展计划(863计划)支持项目(No 2007AA10Z349) （863计划）
