中国药理学通报2011，Vol.27Issue(11)：1510-1514,5.DOI:10.3969/j.issn.1001-1978.2011.11.009
腘窝淋巴结免疫细胞多种表面分子在PLNA评价致敏性中的综合分析
Comprehensive analysis of various popliteal lymph node immune cell surface molecules in allergenic assessment by PLNA
摘要
Abstract
Aim To improve the reliability and sensitivity of popliteal lymph node assay( PLNA )in allergen-ic assessment by analysis of multiple popliteal lymph node( PLN ) immune cells surface molecules. Methods A few female BALB/c mice were divided into four groups and subcutaneously injected with positive drug mercuric chloride ( HgCl2 ), streptozotocin( STZ ), 2, 4,6 - trinitrobenzene sulfonic acid ( TNBS ) and D-penicillamine ( D-pen ) into hind foot respectively. Animals were sacrificed on the 5 th day after injection. Single cell suspensions of injection side of the PLN were prepared. Multiple popliteal lymph node immune cells surface molecules were detected by flow cytometry and then the comprehensive changes were investigated. Results Various significant changes of surface moleules on immune cells were caused by HgCl2 and TNBS,while D-pen and STZ were weaker. Conclusion The changes of the immune cell surface molecules of PLN can reveal the allergenic potential of the test. Because the test causes different types and strength of surface molecular changes , the evaluation of allergenicity should be a comprehensive analysis of changes of cellsurface molecules.关键词
流式细胞术/腘窝淋巴结实验/免疫细胞表面分子/致敏性评价/氯化汞/2,4,6-三硝基苯磺酸/D-青霉胺/链脲佐菌素Key words
flow cytometry/ popliteal lymph node assay/ immune cell surface molecules/ allergenicity assessment/ mercuric chloride/2,4,6-trinitrobenzene sul-fonic acid/ D-Penicillamine/ streptozotocin分类
医药卫生引用本文复制引用
黄路芬,董燕,王青,周联,王培训..腘窝淋巴结免疫细胞多种表面分子在PLNA评价致敏性中的综合分析[J].中国药理学通报,2011,27(11):1510-1514,5.基金项目
广东省"211工程"三期重点学科建设项目(No 粤发改社[2009]972号) （No 粤发改社[2009]972号）
