中国药理学通报2011，Vol.27Issue(11)：1523-1527,5.DOI:10.3969/j.issn.1001-1978.2011.11.012
油茶皂苷通过内质网应激途径诱导人肝癌细胞HepG2凋亡的研究
Study on Youchasaponin-induced apoptosis of HepG2 cells through the endoplasmic reticulum stress
摘要
Abstract
Aim To investigate the inducing effect of Youchasaponin on the apoptosis of human hepatoma carcinoma cell HepG2 in vitro. Methods Effects of Youchasaponin on the proliferation of HepG2 cells were detected by MTT assay; effects on the expression of caspase-3 , PARP, Bip, ATF6, IRE1, Perk, eIF2a and eEF2 were analyzed by Western blot. Results The proliferation of HepG2 cells was significantly inhibited after treatment with Youchasaponin ( 10 ~ 30 mg ?L * ), and meanwhile it activated caspase pathway, induced the fracturing of PARP substrate and furtherly increased the expression of IRE1, and activate Perk, eIF2a, and eEF2. Conclusion Youchasaponin induces the apoptosis of HepG2 through endoplasmic re-ticulum stress pathway.关键词
油茶皂苷/抗肿瘤/人肝癌细胞株(HepG2)/内质网应激/人免疫球蛋白重链结合蛋白(Bip)/RNA激活蛋白激酶的内质网类似激酶/(Perk)Key words
Youchasaponin/ anti-tumor/ human hepatoma carcinoma cell ( HepG2 )/ endoplasmic reticulum stressl heavy-chain binding protein ( Bip )/ Perk分类
医药卫生引用本文复制引用
马丽媛,李林,江玉,史丽颖,冯宝民,王永奇,唐玲..油茶皂苷通过内质网应激途径诱导人肝癌细胞HepG2凋亡的研究[J].中国药理学通报,2011,27(11):1523-1527,5.基金项目
国家自然科学基金资助项目(No 30801509) （No 30801509）
