中国药理学通报2011，Vol.27Issue(11)：1574-1577,4.DOI:10.3969/j.issn.1001-1978.2011.11.023
当归A3活性部位对小鼠巨噬细胞环氧化酶-2活性及基因表达的影响
Effect of angelica A3 active component on activity and expression of cyclooxygenase-2 in mouse RAW264.7 macrophage cells
摘要
Abstract
Aim To investigate effects of angelica A3 active component ( A3 ) on cyclooxygenase-2 ( COX-2 ) activity and expression in lipopolysaccharide ( LPS )-induced mouse RAW264. 7 macrophage cells. Methods Prostaglandin E2( PGE2 ) production and COX-2 enzyme activity were investigated by enzyme-line Im-munosorbnent assay ( ELISA ). RT-PCR and Western blot were used to analyze cyclooxygenase-2 mRNA and the protein expression level. Results A3 20,40, 80mg ? L * was shown to markedly inhibit the LPS-in-duced PGE2 production, COX-2 enzyme activity, COX-2 mRNA and protein expression in RAW264. 7 cells ( P < 0.05 ). Conclusion A3 can directly decrease PGE2 production, which may be related to inhibiting COX-2 gene expression.关键词
当归/A3活性部位/环氧化酶-2/脂多糖/前列腺素E2/巨噬细胞Key words
angelica/ A3 active component/ cyclooxygenase-2 / lipopolysaccharide/ prostaglandin E2/ macrophage cells分类
医药卫生引用本文复制引用
沈建芬,张又枝,肖军花,王嘉陵..当归A3活性部位对小鼠巨噬细胞环氧化酶-2活性及基因表达的影响[J].中国药理学通报,2011,27(11):1574-1577,4.基金项目
国家自然科学基金资助项目(No 30171095) （No 30171095）
