中国药理学通报2011，Vol.27Issue(11)：1582-1585,4.DOI:10.3969/j.issn.1001-1978.2011.11.025
碱性成纤维细胞生长因子单克隆抗体在小鼠体内药代动力学研究
Pharmacokinetics of basic fibroblast growth factor monoclonal antibody in mice
摘要
Abstract
Aim To investigate the preclinical pharma-cokinetics of basic fibroblast growth factor monoclonal antibody ( bFGF-mAb ) in mice. Methods bFGF-mAb was prepared and purified. 1251-bFGF-mAb was labeled by chloramine-T ( Ch-T ), Plasma and tissue were taken at present times. Radioactivity was measured in all samples by trichloroacetic acid ( TCA ) precipitation. Data of plasma concentration were analyzed using 3P97 software. Results Radiochemical purity level of 125I-bFGF-mAb was more than 98% , and trichloroacetic acid ( TCA ) precipitation of 125I-bFGF-mAb was ( 90. 8 ± 10. 2 )%. The main pharmacokinet-ic parameters were as follows: 7j_a( 0. 1 ~ 0. 2 h ), 7j_p ( 1. 05 ~ 1. 84 h ) and 7j_7( 81. 6 ~ 90. 3 h ). Heart, liver and lung were the major organs for deposition of 1251- bFGF-mAb. Conclusion The plasma concentration-time course of 125I-bFGF-mAb is well fitted to three-compartment model, and presents specificity of tissue distribution.关键词
药代动力学/碱性成纤维细胞生长因子/单克隆抗体/同位素示踪法/血药浓度/组织分布/小鼠Key words
pharmacokinetics/ bFGF/ monoclonal antibody / radioisotopic tracing method/ plasma concentration / tissue distribution/ mice分类
医药卫生引用本文复制引用
潘兰红,徐萌,曾世彬,陈文慧,李汉初,盛良翮,朱新海,向军俭,邓宁..碱性成纤维细胞生长因子单克隆抗体在小鼠体内药代动力学研究[J].中国药理学通报,2011,27(11):1582-1585,4.基金项目
国家高技术研究发展计划(863计划)资助项目 (No 2009AA022112) （863计划）
