中国药理学通报2011，Vol.27Issue(11)：1603-1609,7.DOI:10.3969/j.issn.1001-1978.2011.11.031
黄芪甲苷对H2O2致PC12细胞氧化应激损伤的保护作用
A study on protection of astragaioside Ⅳ about oxidative stress on PC12 cells induced by H2O2
摘要
Abstract
Aim To investigate the effect of astragaio-side IV on PC 12 cell injury induced by H2O2 and its underlying mechanism. Methods The viability of PC 12 cells induced by H2O2 was detected by MTT assay. The status of nucleic acid in cell was observed by Hoechst 33258 staining. Apoptosis of PCI2 cells,reactive oxygen species( ROS ) and cell cycle were measured by flow cytometry. The expression of Cyclin Dl, Cyclin A, Phospho-p38 and T-p38 was examined by Western blot. Results The model on oxidative stress of PC 12 cells induced by H2O2 was constructed successfully. The cell viability of PC12 injured by H2O2 was rescued by astragaioside IV, which could inhibitapoptosis of PCI2 cells and reduce ROS and increase percentage of Gj-phase in cell cycle. Furthermore, astragaioside IV could rescue the descending expression of Cyclin Dl and the increasing expression of Phospho-p38 induced by H2O2. Conclusions Astragaioside IV protects PC 12 cells from oxidative stress induced by H2O2 via stimulating the expression of Cyclin Dl and inhibiting active Phospho-p38. The present data suggest that Cyclin Dl and Phospho-p38 might be a target of clinical therapy via antioxidation. It is an insight into prevention of neurodegenerative diseases.关键词
黄芪甲苷/过氧化氢/氧化应激/Cyclin D1/Cyclin A/PC12细胞Key words
astragaioside IV / H2O2 / oxidative stress/ Cyclin Dl/ Cyclin A/PC12 cell分类
医药卫生引用本文复制引用
王世博,邱景富,白群华,李佳佳,和晋渝,高艳军,于超..黄芪甲苷对H2O2致PC12细胞氧化应激损伤的保护作用[J].中国药理学通报,2011,27(11):1603-1609,7.基金项目
重庆市自然科学基金资助项目(No CSTC,2009BA5083) （No CSTC,2009BA5083）
