临床神经病学杂志2011，Vol.24Issue(5)：361-364,4.
粒细胞-巨噬细胞集落刺激因子基因转染的骨髓基质细胞治疗脑梗死的疗效及机制研究
Study of curative effects and the mechanisms of granulocyte-macrophage colony stimulating factor-genetransfected bone marrow stromal cells on treating cerebral infarction
摘要
Abstract
Objective To investigate the curative effects and the mechanisms of granulocyte-macrophage colony stimulating factor (GM-CSF)-gene-transfected bone marrow stromal cells ( BMSCs) on treating cerebral infarction. Methods One hundred and thirty-five SD rats were randomly divided into sham operation group (n = 30), control group (n = 30), BMSCs group (n - 30) and GM-CSF-BMSCs group ( n = 45 ). Middle cerebral artery occlusion ( MCAO) models were made by intraluminal suture method; and 24 h later, the serum-free ot-MEM media, BMSCs and GM-CSF-gene-transfected BMSCs were injected into ischemic boundary zone (IBZ) of rats in control group, BMSCs group and GM-CSF-BMSCs group respectively. At 3 d, 7 d and 21 d after the transplantation, the neurological severity score ( NSS) was detected; and the infarct volume of the model rats and the apoptosis in the IBZ were caculated. The survival and differentiation of transplanted cells in IBZ in GM-CSF-BMSCs group were observed by immunofluroescence technique. Results Compared with the control group, the NSS and percentage of hemispheric lesion volumes ( HLV) of BMSCs group at 21 d after the transplantation were significantly decreased (all P <0. 05) , and the number of apoptosis of BMSCs group at 3 d, 7 d and 21 d after the transplantation were significantly decreased (all P <0. 05); the NSS and HLV of GM-CSF-BMSCs group at 7 d and 21 d after the transplantation were significantly decreased (P < 0. 05 - 0.01), and the number of apoptosis of GM-CSF-BMSCs group at 3 d, 7 d and 21 d after the transplantation were significantly decreased (all P<0.01). Compared with the BMSCs group, the NSS of GM-CSF-BMSCs group at 7 d and 21 d after the transplantation were significantly decreased (all P <0. 05); the HLV of GM-CSF-BMSCs group at 21 d after the transplantation was significantly decreased ( P < 0. 05); the number of apoptosis of GM-CSF-BMSCs group at 3 d, 7 d and 21 d after the transplantation were significantly decreased (all P <0.05). The transplanted cells survived in IBZ and expressed the neuronal marker NeuN in GM-CSF-BMSCs group. Conclusion The curative effects of GM-CSF-gene-transfected BMSCs therapy on cerebral infarction is quite well, which may be related to the anti-apoptotic, nerve replancement and protection.关键词
粒细胞-巨噬细胞集落刺激因子/骨髓基质细胞/基因转染Key words
granulocyte-macrophage colony-stimulating factor/ bone marrow stromal cells/ gene transfection分类
医药卫生引用本文复制引用
林兴建,张颖冬..粒细胞-巨噬细胞集落刺激因子基因转染的骨髓基质细胞治疗脑梗死的疗效及机制研究[J].临床神经病学杂志,2011,24(5):361-364,4.基金项目
江苏省卫生厅“135 工程”重点人才基金资助 （）
