广东医学2011，Vol.32Issue(22)：2897-2899,3.
急性等容血液稀释对兔局灶性脑缺血再灌注损伤的影响
The effect of acute isovolemic hemodilution on reperfusion injury of focal brain ischemia in rabbits
摘要
Abstract
Objective To investigate the effect of mild acute isovolemic hemodilution on VBL, S100 - (3 and TNF -α after reperfusion of focal brain ischemia in rabbits. Methods Eighteen New Zealand rabbits were randomly divided into 3 groups ( n = 6 ): sham operated group ( Group S ), ischemia - reperfusion group ( Group IR ), and hemodilution -ischemia -reperfusion group ( Group HIR ). The right middle cerebral artery embolism model was constructed according to Longa's method. Rabbits in Group S only received angiodiastasis as sham operation. Ischemia was created by embolism for 2 hours followed by reperfusion in rabbits of Group IR, while pre - dilution with 6% hetastarch ( 130/0. 4 ) until Hct reached 30% 20 min before embolism were applied in rabbits of Group HIR. VBL, S100 - (3 ,TNF - α were measured at baseline ( T0 ), 1 h after ischemia ( T1 ), and 1 h ( T2 ), 2 h ( T3 ), and 4 h after reperfusion ( T4 ). Results The levels of VBL and S100 - β at T1 ,T2 ,T3 ,T4 were significantly increased compared with those at T0 in Group IR and HIR ( P < 0. 05 ), with significantly more prominence in group IR ( P < 0. 05 ). The concentrations of TNF - α at T1 , T2 , T3 , T4 were significantly higher than those at T0 in all 3 groups ( P < 0. 05, P < 0. 01 ), with significantly most prominence in Group IR, following with that in Group HIR ( P <0. 05 ). Conclusion Mild acute isovolemic hemodilution benefits the oxygen supply for brain tissue, reduces inflammatory reaction, and alleviates secondary brain injury after brain ischemia -reperfusion in rabbits.关键词
血液稀释/缺血再灌注损伤/S100-β蛋白/肿瘤坏死因子Key words
hemodilution/ ischemic reperfusion injury/ S100 - β protein/ tumor necrosis factor引用本文复制引用
黄焕森,郑进,何雁冰,陈海铭,胡春旭..急性等容血液稀释对兔局灶性脑缺血再灌注损伤的影响[J].广东医学,2011,32(22):2897-2899,3.基金项目
广东省科技计划项目(编号:2010B060900089) （编号:2010B060900089）
