中国人兽共患病学报2011，Vol.27Issue(9)：820-824,5.
结核分枝杆菌广泛耐药株与耐多药株菌体蛋白质组学比较研究
Proteomics comparison of XDR and MDR Microbacterium tuberculosis
摘要
Abstract
The aim was to compare and analyze the protein expression profiles of extensively drug-resistant (XDR) and multidrug-resistant(MDR) tuberculosis isolates, and identify differentially expressed proteins related to XDR-TB. Total proteins extracted from clinical isolates of XDR-TB and MDR-TB as well as the standard mycobacterium tuberculosis H37Rv were analyzed with two-dimensional electrophoresis (2-DE). The Progenesis SameSpots program (Nonlinear Dynamics Limited, U-nited Kingdom) was used for analyzing the 2-DE images to identify differentially expressed proteins in XDR-TB as compared to MDR-TB and the standard strain, and some selected protein spots were used for the MALDI-MS peptide mass fingerprinting. The results of this study showed that protein expression profile of XDR-TB was significantly different from those of MDR-TB and the H37Rv standard strain. There were twenty-eight differentially expressed protein spots in XDR-TB. Fifteen proteins differentially expressed in XDR-TB were identified by MALDI-MS peptide mass fingerprinting, Protein expression profile of XDR-TB is different from those of MDR-TB and the H37Rv standard strain, and the differentially expressed proteins could be used as potential target proteins for future investigations to study their functions in XDR-TB.关键词
广泛耐药结核分枝杆菌/耐多药结核分枝杆菌/蛋白质组学/双向电泳/质谱鉴定Key words
XDR Micrnhacterium tuberculosis/ MDR Microbacterium tuberculosis/ proteomics/ two-dimensional electrophoresis/ MALDI-MS peptide mass fingerprinting分类
医药卫生引用本文复制引用
王盛兰,陈玲,张建勇,宗兆靖,王宝林,李娜娜,王建华,张泓..结核分枝杆菌广泛耐药株与耐多药株菌体蛋白质组学比较研究[J].中国人兽共患病学报,2011,27(9):820-824,5.基金项目
贵州省国际科技合作计划项目(黔科合外G字2009700120)和遵义医学院博士科技启动项目(F 327)联合资助 （黔科合外G字2009700120）
