国际口腔医学杂志2011，Vol.38Issue(4)：388-391,4.DOI:10.3969/j.issn.1673-5749.2011.04.005
碱性磷酸酶mRNA在体内组织工程骨中的表达
Expression of alkaline phosphatase mRNA of tissue engineering bone in vivo
摘要
Abstract
Objective To detect the expression of alkaline phosphatase (akp) mRNA in in vivo tissue engineering bone constructed with calcium phosphate cement and do a comparison with that in naturally healing bone. Methods 28 porous calcium phosphate cement cylinders were implanted into the dorsal muscles of 7 dogs respectively and one molar was pulled out in each dog to create naturally healing bone model. The in vivo tissue engineering bone and the naturally healing bone were harvested at 3/7, 1, 2, 4, 8, 12 and 24 weeks post-implantation. Then, akp mRNA was detected by real-time quantitative polymerase chain reaction (PCR). Results In this experiment akp mRNA was expressed at lower levels with delayed peak in in vivo tissue engineering bone than that in naturally healing bone. However, the expression of akp mRNA shared a. Similar pattern of initially upregu-lated and finally down-regulated in the two kinds of bone. Conclusion The in vivo tissue engineering bone is calcified later and fewer than natrually healing bone. However, the gene expression pattern is similar to that of naturally healing bone in bone formation.关键词
碱性磷酸酶；体内组织工程骨；骨诱导；磷酸钙陶瓷Key words
alkaline phosphatase/ in vino tissue engineering bone/ bone induction/ calcium phosphate cement分类
医药卫生引用本文复制引用
孙兰英,王其宝,包崇云,葛文章,杜毅..碱性磷酸酶mRNA在体内组织工程骨中的表达[J].国际口腔医学杂志,2011,38(4):388-391,4.基金项目
国家自然科学基金资助项目(30672337，30970728) （30672337，30970728）
