国际口腔医学杂志2011，Vol.38Issue(4)：399-402,4.DOI:10.3969/j.issn.1673-5749.2011.04.008
链球菌和放线菌的胞外代谢物代谢组学鉴定
Application of metabonomics for analysis of extracellar metabolites of Streptococcus and Actinomycetes
摘要
Abstract
Objective To investigate the application of metabonomics in research of oral bacteria, through detecting different of the extracellular metabolites, and find the characteristic metabolic profile of each oral bacteria. Methods 'H-nuclear magnetic resonance (NMR) spectra were obtained from each microbial culture at late exponential growth phase. The obtained data were processed by both the unsupervised principal component analysis (PCA) and the supervised partial least squares discriminant analysis (PLS-DA) to find out the differences among the three groups. Results These 1H-NMR spectrograms of each bacteria are obviously different. The result of pattern recongnition presented marked clustering between the three group. PLS-DA analysis has the better effect to different these samples. Conclusion The results indicated that the 1H-NMR-based extracellular metabonomic approach is a feasible and efficient method for differentiating and analyzing oral bacteria.关键词
代谢组学；磁共振；模式识别；口腔细菌Key words
metabonomics/ nuclear resonance/ pattern recognition/ oral bacteria分类
医药卫生引用本文复制引用
袁艺,肖丽英,郭强,李明云,熊萍,李继遥,朱硃,李燕,龚其美,肖晓蓉..链球菌和放线菌的胞外代谢物代谢组学鉴定[J].国际口腔医学杂志,2011,38(4):399-402,4.基金项目
教育部科学技术研究重大基金资助项目(307022)；四川省应用基础研究基金资助项目(07JY029-072) （307022）
