山东农业科学Issue(6)：14-16,19,4.
转基因玉米PCR检测体系的优化研究
Optimization of PCR System for Transgenic Maize
摘要
Abstract
With the transgenic maize as experiment material, the PCR system for detecting exogenous gene was optimized in extracted DNA quality, Mg2 + concentration, annealing temperature and primer dosage. In the optimized system, the dosage of 25 mmol/L Mg2 + was reduced from 1. 5 μl to 1. 2 μl; the dosage of 12.5 mmol/L primer was reduced from 1 μl to 0.5 μl; the annealing temperature was increased by 2℃. The Touchdown PCR reaction conditions were 95 ℃ for 5 min; 95℃ for 1 min, 62.5℃ for 1 min (1℃ decreased after each cycle), 72℃ for 1 min, 15 cycles; 95℃ for 1 min, 57.5℃ for 1 min, 72℃ for 1 min, 20 cycles; and 72℃ for 7 min for final extension.关键词
转基因玉米；RCR扩增；反应体系Key words
Transgenic maize/PCR amplification/Reaction system分类
生物科学引用本文复制引用
何春梅,张成华,刘强,董瑞,高新学,汪黎明,刘铁山..转基因玉米PCR检测体系的优化研究[J].山东农业科学,2011,(6):14-16,19,4.基金项目
国家科技支撑计划项目(2007BAD31B03)、山东省良种工程项目资助. （2007BAD31B03）
