山东科学2011，Vol.24Issue(3)：47-50,4.
废弃烟叶中茄尼醇对照品的制备
Preparation of reference material of solanesol from waste tobacco leaves
摘要
Abstract
This paper is to establish an approach to separate high-purity reference material of solanesol from tobacoo leaves. We initially obtained crude reference material of solanesol with petroleum ether extraction, dynamic saponification and silica gel chromatography. We then employed preparative high-performance liquid chromatography whose ingredient ratio was 60% methanol and 40% ethanol to refine and purify the acquired crude reference material of solanesol. The purified product whose structure was identified with ESI-MS, IR 1H-NMR and 13C-NMR and whose purity of 99. 7% was determined with HPLC satisfied the requirement of reference material. Such an approach is simple, rapid and reproductive and its product has quite high purity so it is suitable for the preparation of reference material of solanesol.关键词
废弃烟叶；茄尼醇；制备Key words
waste tobacoo leaves/ solanesol/ preparation分类
医药卫生引用本文复制引用
舒希凯,林小静,王岱杰,刘代成,耿岩玲,刘静,王晓..废弃烟叶中茄尼醇对照品的制备[J].山东科学,2011,24(3):47-50,4.基金项目
山东省科技攻关项目(2007GG20009005)；济南市高等院校自主创新计划(201004010)；济南市留学人员创业计划(20100409) （2007GG20009005）
