山东农业科学Issue(6)：99-103,5.
转鲑鱼降钙素基因酵母对体外培养破骨细胞的影响
Effect of Recombinant Saccharomyces cerevisiae Expressing Salmon Calcitonin on Osteoclast Culture in vitro
摘要
Abstract
The osteoclasts of new born Wistar mice were obtained by mechanical separation. The recom binant Saccharomyces cerevisiae expressing salmon calcitonin and restricted by enterokinase and Miacalcic were added into the culture solution respectively. The osteoclasts were observed by HE staining. After cultured for seven days, the area of bone resorption pits was analyzed by Image - Pro Plus image software. The results showed that the recombinant Saccharomyces cerevisiae expressing salmon calcitonin had obvious inhibitory ac tion to the absorption function of osteoclasts.关键词
鲑鱼降钙素；破骨细胞；细胞培养Key words
Salmon calcitonin/Osteoclast/Cell culture分类
医药卫生引用本文复制引用
姜勇,张辉,王鹏,赵喜喜,徐科凤,王健,张学成..转鲑鱼降钙素基因酵母对体外培养破骨细胞的影响[J].山东农业科学,2011,(6):99-103,5.基金项目
国家“863”高技术发展与研究计划项目(819-04-10)资助. （819-04-10）
