南方农业学报2011，Vol.42Issue(7)：700-704,5.
不同激素组合对番茄温敏核不育系及其杂交组合花愈伤组织诱导效应
Effects of different hormone combinations on the callus induction in anthers taken from tomato thermo-sensitive genic male sterile lines and their hybrid combinations
摘要
Abstract
[Objective]The present experiment was aimed to study the effects of different hormone combinations on callus induction in anthers taken from tomato thermo-sensitive genic male sterile lines (TSGMSLs) and their hybrid combinations. [Method]Tomato TSGMSLs,viz.,Da 107 and Di D4, and their hybrid combinations, viz., Da 107xNiufanqie and Di D4xHongkunlun were used in the studies. Combinations of different hormones (CPPU, TDZ, KT) in basic MS medium (containing 1.0 mg/L 2,4-D+l.0 mg/L 6-BA) were used for anther callus induction. [Result]Adding different CPPU, TDZ or KT concentrations in basic MS medium significantly improved the induction of anther callus, and the suitable concentration of CPPU, TDZ and KT was 0.4-0.6, 0.03 and 1.5 mg/L, respectively. Under the same hormone combination conditions, the anthers taken from tomato TSGMSLs showed better callus induction compared to those taken from hybrid combinations, the order of the average rate of callus induction and average diameter of callus was: Di D4>Da 107>Di D4x Hongkunlun>Da 107xNiufanqie. [Conclusion]Anthers of different tomato genotypes differed in their sensitivities to different hormones. Furthermore, different combinations of hormones also differed in affecting induction of tomato anther callus, out of them, the combinations of KT and 2.4-D+6-BA showed best results.关键词
番茄/温敏核不育系/杂交组合/花药培养/激素组合/愈伤组织诱导Key words
tomato/ thermo-sensitive genie male sterile lines(TGMSLs)/ anther culture/ hormone combination/ callus induction分类
农业科技引用本文复制引用
韦鹏霄,张例,王茂昌,岑秀芬,王先裕,贺贵柏,韦德斌..不同激素组合对番茄温敏核不育系及其杂交组合花愈伤组织诱导效应[J].南方农业学报,2011,42(7):700-704,5.基金项目
国家科技部重点国际合作项目(2006DFA33380) （2006DFA33380）
广西自然科学基金项目(桂科自0991292) （桂科自0991292）
