南方农业学报2011，Vol.42Issue(7)：708-711,4.
三褶虾脊兰无菌播种快繁技术研究
A rapid micro propagation technology for Calanthe triplicate and generation of aseptic seedlings
摘要
Abstract
[Objective]The experiment was aimed to establish the system of aseptic sowing and rapid propagation technology for Calanthe triplicate. [ Method ]The Calanthe triplicate seeds were used as explants to study the effects of different hormone combinations (0.5 mg/L 6-BA, 0.1 mg/L NAA and 1.0 g/L AC), additive combinations (coconut milk, mashed potato and mashed banana) and different culture media (Hyponex No.l, 1/2 Hyponex No. 1, MS and 1/2MS medium) on seed germination, induction, multiplication, and differentiation and protocorm rooting by the way of seed→protocorm→the whole plant. [Result]After 150 d of culture, the seeds were found to germinate and developed into protocorm with above 80% germination rate in 1/2 Hyponex No.l medium containing 0.5 mg/L 6-BA +0.1 mg/L NAA + 200 mL/L CM + 1.0 g/L AC + 20.0 g/L sugar. The induced protocorm showed best multiplication (multiplication coefficient 4.5) in 1/2MS medium containing 100 ml/L CM + 2.0 mg/L 6-BA + 0.1 mg/L NAA + 1.0 g/L AC + 20.0 g/L sugar. After sub-culturing, the protocorm in Hyponex No.l medium (containing 0.1 mg/L 6-BA + 0.1 mg/L NAA + 100.0 g/L mashed potato +1.0 g/L AC + 20 g/L sugar) for 40 days and then transferring subculture into Hyponex No.l medium (containing 0.1 mg/L NAA + 100.0 g/L mashed potato + 2.0 g/L AC + 20.0 g/L sugar) for 40 days, the young plantlets were induced and were able to develop into the whole plant. [Conclusion]The technology for rapid propagation of Calanthe triplicate was established and aseptic seedlings were transplanted successfully which showed good growth with high vigor. The technology developed will help in protecting germplasm resources, seedling propagation and its industrial applications.关键词
三褶虾脊兰/无菌播种/原球茎/快繁技术Key words
Calanthe triplicate/ aseptic sowing/ protocorm/ rapid propagation technology分类
农业科技引用本文复制引用
罗远华,黄敏玲,叶秀仙,林兵..三褶虾脊兰无菌播种快繁技术研究[J].南方农业学报,2011,42(7):708-711,4.基金项目
福建省科技重大专项项目(2010NZ003) （2010NZ003）
福建省科技计划重点项目(2008NO115) （2008NO115）
福建省农业科学院科技创新团队建设基金项目(STIF-Y06) （STIF-Y06）
