西北国防医学杂志2011，Vol.32Issue(5)：335-337,3.
人hTERT基因的pCIneo真核表达载体的构建
Construction of pCIneo eukaryotic expression plasmid of hTERT
摘要
Abstract
Objective: To construct pCIneo eukaryotic expression plasmid of hTERT. Methods: The primers were designed according to hTERT mRNA sequence and the hTERT gene was amplified by RT - PCR using 293 cells as template. Then gene of hTERT was subcloned into vectors pCIneo and confirmed by DNA sequence analysis and restriction endonuclease digestion. Results; The analysis result of recombinant vector by restriction endonucle-ase digestion showed that hTERT gene has been correctly inserted into plasmid pCIneo. The homology of hTERT fragment was 99. 89 % in nucleotide acid,and 100 % in amino acid compared with that in GenBank. Conclusion; The successful construction of the pCIneo eukaryotic expression plasmid of hTERT laid a solid foundation for its further research.关键词
人端粒酶逆转录酶/表达载体/基因转染Key words
Human telomerase reverse transcriptase/ Expression vector/ Gene transfection分类
生物科学引用本文复制引用
滕勇,李旭升,关玉成,李雁军..人hTERT基因的pCIneo真核表达载体的构建[J].西北国防医学杂志,2011,32(5):335-337,3.基金项目
兰州军区医药卫生科研计划资助项目(LXH2007014) （LXH2007014）
