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SSR标记技术在玉米品种“先玉335”真伪性鉴定中的应用

岳静 朱志成 高洁 李洪建

吉林农业大学学报2011,Vol.33Issue(5):507-510,4.
吉林农业大学学报2011,Vol.33Issue(5):507-510,4.DOI:22-1100/S.20110706.1419.002

SSR标记技术在玉米品种“先玉335”真伪性鉴定中的应用

Application of SSR Markers in the Identification of Maize Xianyu 335

岳静 1朱志成 2高洁 3李洪建2

作者信息

  • 1. 沈阳化工大学环境与生物工程学院,沈阳110142
  • 2. 辽宁省种子管理局,沈阳110034
  • 3. 吉林农业大学农学院,长春130118
  • 折叠

摘要

Abstract

In this study, 20 pairs of SSR basic core primers and 20 pairs of SSR extended core primers were used to amplify for identifying the authenticity of maize varieties by SSR markers. The differences between the samples tested and the standard sample (xianyu 335) were analyzed at the DNA level. The results showed that sample No. 1 had obvious differences with the standard sample (xianyu 335) , so sample No. 1 was not xianyu 335. However sample No. 2 had no obvious differences with the standard sample (xianyu 335), it was surely xianyu 335. SSR markers could be fast, accurate and efficient to I-dentify the authenticity of maize varieties.

关键词

玉米/SSR标记/品种鉴定/先玉335

Key words

maize ( Zea mays L.)/ simple sequence repeat marker/ variety identification/ xianyu 335

分类

农业科技

引用本文复制引用

岳静,朱志成,高洁,李洪建..SSR标记技术在玉米品种“先玉335”真伪性鉴定中的应用[J].吉林农业大学学报,2011,33(5):507-510,4.

基金项目

辽宁省农村经济委员会专项(2010-06) (2010-06)

吉林农业大学学报

OA北大核心CSCDCSTPCD

1000-5684

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