应用生态学报2011，Vol.22Issue(9)：2316-2324,9.
地表球囊霉诱发番茄抗早疫病的机理
Mechanism of tomato plants enhanced disease resistance against early blight primed by arbuscular mycorrhizal fungus Glomus versiforme
摘要
Abstract
Arbuscular mycorrhiza (AM) can not only improve host plants nutrient absorption, but also enhance their disease resistance. Taking the tomato (Lycopersicon esculentum) seedlings pre-inoculated with arbuscular mycorrhizal fungus (AMF) Glomus versiforme as test materials, this pa-per studied their protective enzyme activities and defense-related genes expression, and their resist-ance against a fungal pathogen Alternaria solani Sorauer which causes early blight. The seedlings pre-inoculated with AMF and later inoculated with A. solani showed significantly higher activities of superoxide dismutase (SOD) and peroxidase (POD) in leaves. The leaf SOD activity of the dually inoculated plants reached the maximum 18 h after pathogen inoculation, being 28. 6% , 79. 2% , and 82. 8% higher than that of the plants with G. versiforme inoculation alone, pathogen inoculation alone, and non-inoculation, and the leaf POD activity reached the maximum 65 h after pathogen in-oculation, being 762% , 18.3%, and 1710% higher, respectively. Real time RT-PCR analysis showed that dual inoculation with G. versiforme and A. solani strongly induced the expression of three defense-related genes. The transcript levels of pathogen-related protein (PR1), basic type β-1,3-glucanase (PR-2) , and chitinase (PR-3) in leaves were 9. 67-, 8. 54-, and 13. 4-fold higher, as compared with the non-inoculation control, respectively. Bioassay showed that the disease inci-dence and disease index of the seedlings pre-inoculated with G. versiforme were reduced by 36. 3% and 61.4% , respectively, as compared with the non-mycorrhizal control plants. These findings in-dicated that mycorrhizal colonization could induce stronger and quicker defense responses of host to-mato plants, and priming could be an important mechanism of the enhanced disease resistance of mycorrhizal tomato plants.关键词
丛枝菌根真菌/番茄/诱发/早疫病/抗病性Key words
arbuscular mycorrhizal fungus/ tomato/ priming/ early blight/ disease resistance.分类
农业科技引用本文复制引用
宋圆圆,王瑞龙,魏晓晨,卢永键,唐朝阳,吴国昭,苏贻娟,曾任森..地表球囊霉诱发番茄抗早疫病的机理[J].应用生态学报,2011,22(9):2316-2324,9.基金项目
国家自然科学基金项目(30670331,31070388)资助. （30670331,31070388）
