水生生物学报2011，Vol.35Issue(5)：727-738,12.DOI:10.3724/SP.J.1035.2011.00727
生长激素受体信号相关因子在斑马鱼中的比较表达分析及GHR信号通路体内研究模型的建立
COMPARATIVE EXPRESSION ANALYSIS OF GHR SIGNALING RELATED FACTORS IN ZEBRAFISH (DANIO RERIO) AND AN IN VIVO MODEL TO STUDY GHR SIGNALING
摘要
Abstract
Growth hormone receptor (GHR) signaling pathway plays an important role in postnatal growth of animals.Although significant progress has been made in elucidating the signaling pathways activated by GHR in recent years,a comparative expression analysis of all the GHR signaling related genes and evaluation of GH-signal activation (GHSA)in an in vivo model still remain elusive.The zebrafish (Danio rerio) is an excellent model organism to study both developmental and physiological processes.In the present study,we comparatively analyzed the expression of GHR signal related genes,such as gh,ghra,ghrb,jak2a,jak2b,stat5.1,stat5.2,igf1,c-fos,socsl and socs2 in adult tissues,and during embryonic development by means of RT-PCR.Maternal expression was found in most of them and their zygotic expression mostly started from early-somite stage,far before the differentiation of pituitary somatotrophs and before the establishment of a functional circulatory system.In adults,the tissue distribution abundance of socs mRNA was negatively correlated with the GH-signal targets,igfl and c-f os.By analyzing the transcription level ofc-fos and igfl and the promoter activity of spi2.1,in zebrafish embryos injected with different constructs,i.e.,the expression constructs of gh and ghr,we established an in vivo model to evaluate GH-signal activation (GHSA) in early development of zebrafish embryos.Overexpression of gh or ghr alone in zebrafish embryos could significantly stimulate GHSA at 1 day post-fertilization (dpf) and 3 dpf,strongly indicating the existence of functional GH and GHR proteins in zebrafish embryos from 1 dpf,far prior to the formation of functional pituitary gland.A synergetic overexpression of gh and ghr could amplify the GHSA effects ofgh or ghr overexpression.关键词
生长激素/生长激素受体/GHR信号通路/斑马鱼/转录分析Key words
GH/GHR/GHR signaling/Zebrafish/Transcriptional analysis分类
生物科学引用本文复制引用
Abu Shufian Ishtiaq Ahmed,于力群,朱作言,孙永华..生长激素受体信号相关因子在斑马鱼中的比较表达分析及GHR信号通路体内研究模型的建立[J].水生生物学报,2011,35(5):727-738,12.基金项目
The National Natural Science Foundation of China (30972248) and the Youth Sunshine Project of Wuhan City （30972248）
