解剖学杂志2011，Vol.34Issue(5)：584-587,4.
多种细胞因子诱导大鼠间充质干细胞向多巴胺能神经元分化
Some cytokines induce rat bone marrow mesenchymal stem cells to differentiate into dopaminergic neurons in vitro
摘要
Abstract
Objective: To explore the feasibility that various cytokines, including sonic hedgehog (SHH), basic fibroblast growth factor (bFGF), All-trans retinoic acid (ATRA), glial-derived neurotrophic factor (GDNF) induce the rat bone marrow mesenchymal stem cells into dopaminergic neurons, and the functions of induced cells. Methods: The BMSCs derived from rat bone marrow were cultured and maintained in vitro. After being induced by basic fibroblast growth factor (bFGF) for 24 h, some BMSCs were incubated with all-trans retinoic acid (ATRA) and glial-derived neurotrophic factor (GDNF) for 4 d, and some BMSCs were incubated with sonic hedgehog (SHH) and fibroblast growth factor-8 (FGF-8) for 12 d. Then the expressions of nestin, microtubule-associated protein- Ⅱ (MAP-Ⅱ ) and tyrosine hydroxylase (TH) were detected by immunocytochemical methods and the content of catecholamine (CA) in supernatant fluid were determined by fluorescent analysis, respectively. Results: Under the inverted phase contrast microscope, the induced BMSCs by two induced methods showed typical neuronal morphological characteristics after induction. The expression rates of nestin, MAP- Ⅱ and TH were (48.1±3.2)%, <63.2±2.9)%, (30. 7±5. 9)% induced by bFGF+ATRA+GDNF and (54. 7±4. 1)%, (75.8±5.2)%, (46. 7±3. 3)% and by bFGF+SHH+FGF8, respectively. The expression rate of TH of the cells induced by bFGF+SHH +FGF8 was higher than that of the cells induced by bFGF+ATRA+GDNF (P<0. 01). The content of CA in supernatant fluid was (2. 731±0. 81)fxg/L and (2. 977±0. 16)μg/L, respectively induced by different cytokines. Conclusion: bFGF+ SHH+FGF8 cytokines group could induce rat BMSCs to differentiate into dopaminergic neuron-like cells more effectively.关键词
间充质干细胞/神经元/细胞分化/细胞因子/大鼠Key words
mesenchymal stem cells/neuron/cell differentiation/cytokines/rat引用本文复制引用
陈丹丹,付文玉,庄文欣,吕娥,郭健,李锋杰..多种细胞因子诱导大鼠间充质干细胞向多巴胺能神经元分化[J].解剖学杂志,2011,34(5):584-587,4.基金项目
山东省自然科学基金(Y2003C01 （）
Y2008C129) （）
留学回国人员科研启动基金(教外司留[2009]1001号) （教外司留[2009]1001号）
