广西医科大学学报2011，Vol.28Issue(4)：511-514,4.
T细胞非霍奇金淋巴瘤微卫星不稳定性及hMSH2、hMLH1蛋白表达研究
STUDY OF MICROSATELLITE DNA INSTABILITY AND hMSH2, hMLH1 PROTEIN EXPRESSION IN T-CELL LYMPHOMA
摘要
Abstract
Objective:To study the role of the microsa-tellite instability and the defects of mismatch repair system genes in T-cell Non-Hodgkins lymphoma. Methods: We detected microsatellite instability at 4 microsatellite loci (D17S945,D17S938, D17S947 and D17S926) around the p53 gene on chromosome 17 by PCR, polyacrylamide gel electrophoresis and silver staining methods and analyzed hMSH2, hMLHl protein expression by immunohistochemistry in 38 cases of T-cell Non-Hodgkins lymphoma. Results:The positive rate of MSI was 23. 68% (9/38), the rate of LOH was 7. 89% (3/38). At the 4 mirosatellite loci, frequency of MSI and LOH varied from 2. 86% to 18. 42%, and from 0 to 5. 26%, respectively. No significant difference was found in MSI incidence between different mirosatellite loci( P ＞0. 05). The absent expression rate of hMSH2, hMLHl prontein was 52. 63% (20/38)and 42. 11%(16/38), respectively, in tumor tissues. Conclusion: MSI and the absent expression of mismatch repair genes are present in T-cell Non-Hodgkin's lymphoma.关键词
非霍奇金淋巴瘤/T细胞/微卫星不稳定性/hMSH2/hMLH1Key words
Non-Hodgkin's lymphoma/ T-cell/ microsatellite instability/ hMSH2/ hMLH1分类
医药卫生引用本文复制引用
罗春英,邓飞,黄照权,卢运龙,黄永秩..T细胞非霍奇金淋巴瘤微卫星不稳定性及hMSH2、hMLH1蛋白表达研究[J].广西医科大学学报,2011,28(4):511-514,4.基金项目
广西教育厅科研基金资助项目(No.200911LX337) （No.200911LX337）
广西右江民族医学院项目(No.右医科字[2007]2号) （No.右医科字[2007]2号）
