江西农业大学学报2011，Vol.33Issue(3)：514-520,7.
油茶FBPase基因的全长cDNA克隆及序列分析
Cloning and Sequence Analysis of FBPase Gene from Camellia oleifera
摘要
Abstract
FBPase is one of the key enzymes controlling Calvin Cycle, Which helps to improve Clavin circulation to accelerate photosynthetic efficiency of plants. The full length cDNA of FBPase gene was separated from the EST library and the cDNA library of Camellia oleifera by the method of 5' RACE and 3' RACE. The results showed that this gene is 1 356 bp in length, and it has an open reading frame of 1 020 bp, and the a-lignment of nucleotide acids sequence reveals that the comparability of Camellia oleifera and populus trichocar-pa is 84%. This gene encodes 339 aminoacids, the protein is a stable protein whose Isoelectric point is 5.54. Camellia oleifera FBPase encoded protein does not contain disulfide, thus the gene is c-FBPase named co -fbp. This study is significant help to the research on the photosynthesis of Camellia oleifera, increaseing energy efficiency and production.关键词
油茶/FBPase基因/基因克隆/全长cDNA/序列分析Key words
Camellia oleifera/FBPase gene/gene cloning/full-length cDNA/sequence analysis分类
农业科技引用本文复制引用
谭晓风,曹彦妮,郭静怡,刘凯..油茶FBPase基因的全长cDNA克隆及序列分析[J].江西农业大学学报,2011,33(3):514-520,7.基金项目
国家自然科学基金项目(31070603) （31070603）
