江西农业大学学报2011，Vol.33Issue(3)：564-567,588,5.
鸡NCOA1基因发育性表达模式的研究
Developmental Expression Patterns of NCOA1 Gene in Chicken
摘要
Abstract
The paper studieds the populations with different reproduction performance including female line (S3), male line (F, D, Y) for identification of the relative expression levels and expression patterns of NCOAl gene changes in hypothalamus, pituitary, ovary, blood and liver in different development periods by using real-time quantitative PCR. The results indicated that the NCOAl gene expression level in gonad tissues was higher than that in other tissues. The mRNA expression regulation of NCOAl gene was similar to the growth regulation of chicken gonad tissues and egg - laying. NCOAl gene expression increased along with growth with undulatory property, and there was little expression before 14 weeks. NCOAl gene expression rapidly increased along with sex maturation, and high expression was found at 28 weeks then decreased. NCOAl expression level of the female line was earlier than that of the male line and had significantly higher mRNA expression after 14 weeks ( P < 0. 05 ). NCOAl genes may be playing an important role in reproductive process. These results suggested that the mRNA level of NCOAl gene in gonad tissues was different in postnatal developmental stages among lines, which provided some data for further study on the NCOAl gene for elucidating the molecular mechanism of reproduction traits.关键词
鸡/实时荧光定量PCR/NCOA1基因/表达模式Key words
chicken/ real -time quantitative PCR/ NCOAl gene/ expression pattern分类
农业科技引用本文复制引用
赵振华,黎寿丰,黄华云,李春苗,张静,薛龙岗,丁余荣..鸡NCOA1基因发育性表达模式的研究[J].江西农业大学学报,2011,33(3):564-567,588,5.基金项目
江苏省“333”工程培养资金项目(BRA2010162),现代农业产业技术体系建设专项资金,中国农业科学院博士基金(JQ2009002) （BRA2010162）
