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食用菌纤维素内切酶的分子建模与分析

黄婷婷 易卓林 茆灿泉

江西农业大学学报2011,Vol.33Issue(3):584-588,5.
江西农业大学学报2011,Vol.33Issue(3):584-588,5.

食用菌纤维素内切酶的分子建模与分析

Molecular Modeling and Analyses of the Endoglucanases from Edible Fungi

黄婷婷 1易卓林 1茆灿泉1

作者信息

  • 1. 西南交通大学生命科学与工程学院/分子进化与应用生物学实验室,四川成都610031
  • 折叠

摘要

Abstract

SWISS -MODEL and Discovery Studio were used to predict and analyze the tertiary structure of edible fungi endoglucanases. It was found that the endoglucanases of V. Volvacea and T. Hirsuta appear to like a barrel with alpha - helices in their inner layer and beta-folding sheets in its outer layer, with random coils in their two bottoms; as a salt-bond formation center in V. Volvacea endoglucanase, arginninel29 participates in the formation of six salt-bonds; catalytic residues glutamic acid215 and glutamic acid248 in V. Volvacea endoglucanase participate in the formation of five hydrogen bonds, while the number for T. Reesei endoglucanase II is three. Based on the analyses of the linker sequences and catalytic related hydrogen-bond, the authors suggest that ami no acids in these sites might be mutable optimized for V. Volvacea and T. Hirsuta endoglucanases. The work would be helpful for structure optimization and utilization of edible fungi endoglucanases.

关键词

分子建模/纤维素内切酶/食用菌

Key words

molecular modeling, endoglucanase, edible fungi

分类

生物科学

引用本文复制引用

黄婷婷,易卓林,茆灿泉..食用菌纤维素内切酶的分子建模与分析[J].江西农业大学学报,2011,33(3):584-588,5.

基金项目

中央高校基本科研专项资金(SWJTU09ZT28) (SWJTU09ZT28)

江西农业大学学报

OA北大核心CSCDCSTPCD

1000-2286

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