江西农业大学学报2011,Vol.33Issue(3):584-588,5.
食用菌纤维素内切酶的分子建模与分析
Molecular Modeling and Analyses of the Endoglucanases from Edible Fungi
摘要
Abstract
SWISS -MODEL and Discovery Studio were used to predict and analyze the tertiary structure of edible fungi endoglucanases. It was found that the endoglucanases of V. Volvacea and T. Hirsuta appear to like a barrel with alpha - helices in their inner layer and beta-folding sheets in its outer layer, with random coils in their two bottoms; as a salt-bond formation center in V. Volvacea endoglucanase, arginninel29 participates in the formation of six salt-bonds; catalytic residues glutamic acid215 and glutamic acid248 in V. Volvacea endoglucanase participate in the formation of five hydrogen bonds, while the number for T. Reesei endoglucanase II is three. Based on the analyses of the linker sequences and catalytic related hydrogen-bond, the authors suggest that ami no acids in these sites might be mutable optimized for V. Volvacea and T. Hirsuta endoglucanases. The work would be helpful for structure optimization and utilization of edible fungi endoglucanases.关键词
分子建模/纤维素内切酶/食用菌Key words
molecular modeling, endoglucanase, edible fungi分类
生物科学引用本文复制引用
黄婷婷,易卓林,茆灿泉..食用菌纤维素内切酶的分子建模与分析[J].江西农业大学学报,2011,33(3):584-588,5.基金项目
中央高校基本科研专项资金(SWJTU09ZT28) (SWJTU09ZT28)