植物学报2011，Vol.46Issue(5)：569-574,6.DOI:10.3724/SP.J.1259.2011.00569
月季愈伤组织的诱导及植株再生
Callus Induction and Plant Regeneration of Rose
摘要
Abstract
We used the leaves and stems of aseptic seedlings of 5 rose cultivars that can successfully live outside through the winter in Heilongjiang Province as explants to investigate callus induction and plant regeneration. The leaves and stems of the 5 rose cultivars could induce callus; media containing 2,4-D could induce callus better; and a high concentration of cytokinin was not suitable for callus induction. TDZ played an important role in callus differentiation; culture under light conditions was effective for callus differentiation; and callus differentiation decreased with increasing number of subcultures. Plant regeneration could be successfully induced from the callus of 2004-8 and 2004-9 in experimentation, and the callus differentiation rate of the 2 varieties was 45% and 38%, respectively.关键词
愈伤组织诱导/植株再生/月季Key words
callus induction/plant regeneration/rose引用本文复制引用
陈雪,张金柱,潘兵兵,桑成瑾,马雪,杨涛,车代弟..月季愈伤组织的诱导及植株再生[J].植物学报,2011,46(5):569-574,6.基金项目
黑龙江省留学基金(No.LC08C20)和黑龙江省教育厅海外学人科研资助项目(No.1154h04) （No.LC08C20）
