南方医科大学学报Issue(9)：1484-1487,4.
人羊膜上皮细胞干细胞特性及向胰岛素分泌细胞分化的研究
Stem cell characteristics and islet differentiation potential of human amniotic epithelial cells
摘要
Abstract
Objective To establish a method for isolating and culturing human amniotic epithelial cells (hAECs) in a serum-free medium and investigate their transdifferentiation ability into islet-like cells. Methods The culture condition of hAECs was optimized using DMEM with different supplements. The genetic stability of the tenth-passage cells was assessed by chromosome analysis and G-banding method. The stem cell characteristics of the cells were identified by examination of the surface markers using immunofluorescence methods. The endocrine-related genes and hormones of the cells were tested after induced differentiation into islet-like cells. Results The hAECs allow stable passaging in the presence of 10 ng epidermal growth factor (EGF) in the culture medium. After 10 passages, the cells maintained a normal karyotype and G-banding profile. The hAECs expressed many multi-potent stem cell markers, including SSEA4, TRA-1-60, and TRA-1-81. After induced differentiation, the endocrine-related genes were expressed in the islet-like cells, including PDX1, ngn3,. Insulin and glucagon. Insulin secretion increased in the differentiated islet-like cells in response to high glucose exposure. Conclusion We established a method for isolating and expanding the hAECs in a serum-free medium. hAECs possess stem cell characteristics and can be induced to differentiate into islet-like cells in vitro.关键词
人羊膜上皮细胞/胰岛素分泌细胞/横向分化Key words
human amniotic epithelial cells/ islet-like cells/ transdifferentiation分类
医药卫生引用本文复制引用
王建,彭琳,卢光琇..人羊膜上皮细胞干细胞特性及向胰岛素分泌细胞分化的研究[J].南方医科大学学报,2011,(9):1484-1487,4.基金项目
国家自然科学基金(30700272) （30700272）
教育部新教师基金(422201017) （422201017）
湖南省科技计划项目(2009FJ3180)Supported by National Natural Science Foundation of China (30700272). （2009FJ3180）
