南方医科大学学报Issue(9)：1504-1508,5.
二甲双胍对肾癌细胞凋亡的影响及机制
Effect of metformin on apoptosis of renal cell carcinoma cells in vitro and its mechanisms
摘要
Abstract
Objective To evaluate the effect of metformin on the apoptosis of renal cell carcinoma (RCC) cells in vitro and its mechanisms. Methods Fluorescent microscopy and flow cytometry were used to examine the changes in the apoptosis of 786-O cells after metformin treatment. The possible signaling molecules involved in this process were analyzed by immunoblot analysis of AMP-activated protein kinase (AMPK) signaling and caspase 9. Results Metformin induced apoptosis and caspase 9 activation in 786-O cells in low-serum medium but not in normal-serum medium. Metformin also induced AMPK activation in 786-O cells, but this activation was not associated with the cell proliferation inhibition or apoptosis-inducing effect of metformin. Conclusion Metformin can induce apoptosis of RCC cells in vitro, suggesting its potential as a therapeutic agent for RCC.关键词
二甲双胍/肾细胞癌/细胞增殖/细胞凋亡:AMPKKey words
renal cell carcinoma/ metformin/ cell proliferation/ apoptosis/ AMP-activated protein kinase分类
医药卫生引用本文复制引用
李明,刘俊,胡卫列,贾春宏,李海燕,温芝华,邹志鹏,白晓春,罗深秋..二甲双胍对肾癌细胞凋亡的影响及机制[J].南方医科大学学报,2011,(9):1504-1508,5.基金项目
国家自然科学基金(31000633)Supported by National Natural Science Foundation of China(31000633). （31000633）
