南方医科大学学报Issue(9)：1539-1542,4.
核因子KB受体活化因子配体、护骨素在不同月龄雄性大鼠股骨的表达
Expressions of RANK, RANKL, and osteoprotegrin in male rats at different ages
摘要
Abstract
Objective To investigate the expression of receptor activator of nuclear factor-KB (RANK), its ligand RANKL, and osteoprotegrin, and observe the effects of αD3 on their expressions in male rats at different ages. Methods Wistar rats at 6 weeks, 6 months, and 24 months (n=15) were examined for mRNA expressions of RANK/ RANKL and osteoprotegrin in the left proximal femur using RT-PCR and for their protein expressions in the right femur using immunohistochemistry. RANK/RANKL and osteoprotegrin expressions were also detected in another 15 rats aged 24 months following intragastric administration of 0.05 ug/kg αD3 (3 times a week for 10 weeks). Results Compared with 6-week-old rats, 6-month- and 24-month-old rats showed a 6.2-fold and 7.3-fold increase of RANKL mRNA expression, respectively (P<0.05), and osteoprotegrin mRNA levels increased slightly with age. αD3 treatment resulted in significantly increased expression of RANK in 24-month-old rats with a lowered RANKL/osteoprotegrin ratio. RANKL and osteoprotegrin were co-localized in the osteoblasts and chondrocytes. αD3 treatment also caused an increased expression of osteoprotegrin mRNA in 24-month-old rats. Conclusion The age-related increase of the ratio of RANKL/osteoprotegrin mRNA promotes osteoclast activity and bone turnover. αD3 has favorable effect on osteogenesis and suppress bone absorption in the femur possibly by reducing RANK expression and lowering RANKL/osteoprotegrin ratio.关键词
护骨素:核因子KB受体活化因子配体/核因子KB受体活化因子/αD3/雄性Key words
osteoprotegerin/ receptor-activated nuclear factor kappa B/ receptor activated nuclear factor kappa B ligand/ alfacalcidol/ male分类
生物科学引用本文复制引用
周雄文,刘迎春,翦新春,雷勇华,吴英..核因子KB受体活化因子配体、护骨素在不同月龄雄性大鼠股骨的表达[J].南方医科大学学报,2011,(9):1539-1542,4.基金项目
湖南省发改委高技2009(1390) （1390）
湖南省科技厅2009(ck3064) （ck3064）
