RP-HPLC法同时测定藏药珠芽蓼中牡荆素、槲皮苷和槲皮素的含量OA北大核心CSCDCSTPCD
Simultaneous Quantitative Determination of Viterxin, Quercetin and Quercitrin in Polygonum viviparum in Tibet Plateau by RP-HPLC
采用RP-HPLC法建立了同时测定珠芽蓼中牡荆素、槲皮苷、槲皮素三种黄酮的方法,色谱柱为kromasil C18(250 mm×4.6mm,5μm),以甲醇-水(含0.25％磷酸)为流动相梯度洗脱,流速1 mL/min,检测波长360 nm.结果表明,牡荆素、槲皮苷和槲皮素质量浓度在8～40、5～50 μg/mL和5.33～52 μg/mL范围内与色谱峰面积均呈良好的线形关系,平均回收率分别为100.3％、98.9％和100.4％,日内稳定性偏差…查看全部>>
To develop a 8ensitive and specific reversed-phase high perfonnance liquid chromatography ( RP-HPLC) method for simultaneous detennination of three flavoniods in Polygonum viviparum,the HPLC separation was performed on a Kromasil C18 column (250 mm ×4.6 mm,5 μm) and detected with DAD at 360 nm.The gradient elution was carried out with methano-H2O (containing 0.25％ phosphoric acid) as the mobile phase at a flow rate of 1 mL/min.The results indicated …查看全部>>
续艳丽;董琦;胡凤祖
中国科学院西北高原生物研究所,西宁810008中国科学院研究生院,北京100049中国科学院西北高原生物研究所,西宁810008
医药卫生
珠芽蓼牡荆素槲皮苷槲皮素RP-HPLC
Polygonum uiunparumviterxinquercetinquereitrinRP-HPLC
《天然产物研究与开发》 2011 (5)
894-897,4
评论