天然产物研究与开发2011，Vol.23Issue(5)：905-908,969,5.
北虫草体外抗氧化和PC12氧化损伤 修复作用的有效部位的筛选
Antioxidant Activity and Oxidative Injury Protection for PC12 of Three Extracts from Cordyceps militaris Fruiting Bodies in vitro
摘要
Abstract
Cordyceps militaris fruiting bodies were extracted by using the method of systematic solvent extraction, and three different extracts were prepared named chloroform extract,acetic ether extract and ethanol extract,respectively. In vitro,the antioxidant activities of different extracts were investigated by using chemiluminescence measurements,and the activities of oxidative injury protection were assessed by'using PC 12 oxidative injury model induced with H2O2. The su-peroxide free radical (O2~ ' ) system and H2O2 system assays showed that the acetic ether extract had notable antioxidant activity,their scavenging rate for both H2O2 and O2~ ' were highest among the three extracts,whose Icg, were 88.5 p.g/ mL and 190 u.g/mL,respectively. Moreover,the oxidative injury protection for PC12 assay showed that the acetic ether extract had significant rehabilitation effect in comparison with chloroform extract and ethanol extract,and it rehabilitated PC 12 cell treated with H2O2 in a dose-dependent manner. Therefore,it was concluded that the acetic ether extract was the principal extract of both antioxidant and the activity of oxidative injury rehabilitation among three extracts from C. militaris fruiting bodies.关键词
北虫草/抗氧化/PC12细胞/氧化损伤/系统溶剂法Key words
Cordyceps militaris/ antioxidant activity/PC12 cell/oxidative injury/systematic solvent extraction分类
医药卫生引用本文复制引用
杜秀菊,张秀省,张劲松,贾薇,刘艳芳,杨焱,唐庆九,周帅..北虫草体外抗氧化和PC12氧化损伤 修复作用的有效部位的筛选[J].天然产物研究与开发,2011,23(5):905-908,969,5.基金项目
国家科技部支撑基金项目(2006BAD06B08) （2006BAD06B08）
