中国中西医结合杂志Issue(10)：1389-1393,5.
基于均匀设计分析黄芪汤活性组分抗二甲基亚硝胺大鼠肝纤维化的配伍作用
Uniform Designed Research on the Active Ingredients Assembling of Huangqi Decoction for Inhibition of DMN-induced Liver Fibrosis
摘要
Abstract
Objective To screen out effective ingredients of Huangqi Decoction (HQD) on dimethylnitro-samine (DMN) induced liver fibrosis and its assembling actions. Methods (1) DMN solution (0.5%) was peri-toneally injected to rats to prepare the liver fibrosis model for 12 times, starting from the 1st day of modeling to the end of the 4th week. Uniform design method with 4-factor 8-level table was used to optimize the proportion of four ingredients from HQD, including astragalosides (AS), astragalus flavonoids (AF), glycyrrhizae acid (GA), and glycyrrhizae flavonoids (GF). Moreover, the changes of hydroxyproline (Hyp) content in the liver issue and the level of alanine aminotransferase (ALT) in serum were observed as screen indices, and the method of regression analysis was used to find out an optimal combination. (2) A further study for comparing and verifying the efficacy of the obtained optimized prescription was conducted by observing the changes of fibrosis pathology, the content of Hyp in the liver tissue and serum enzyme activity after medication. Results The optimal proportion of AS and GA was 164:48. Compared with the model group, the content of Hyp in the liver tissue and the levels of ALT, as-partate aminotransferase (AST), and alkaline phosphatase (ALP) in serum decreased significantly, indicating the inhibiting effect of HQD and the AS/GA combination group on hepatic fibrosis formation (P<0.05). The AS/GA combination group was better than AS/GA used alone group in reducing the content of Hyp in the liver tissue and the level of ALT in serum. Furthermore, the AS/GA combination group was better than the HQD group in reducing the level of ALT in serum. Conclusions AS and GA were effective ingredients of HQD, and the combination of AS and GA had obvious synergistic effect in reducing liver collagen deposition and decreasing serum ALT activity in DMN-induced liver fibrosis.关键词
肝纤维化/均匀设计/黄芪汤/中药组分配伍Key words
cirrhosis/ uniform design/ Huangqi Decoction/ combination components引用本文复制引用
仝欣,刘成海,胡义扬,徐列明,刘平,陈高峰,陆雁,宁冰冰,刘佳,李风华,李医明,郑青山,孙明瑜..基于均匀设计分析黄芪汤活性组分抗二甲基亚硝胺大鼠肝纤维化的配伍作用[J].中国中西医结合杂志,2011,(10):1389-1393,5.基金项目
国家重点基础研究发展计划项目资助(973计划,No.2006CB504801) （973计划,No.2006CB504801）
国家自然科学基金重大研究计划重点项目(No.90409020) （No.90409020）
上海市医学领军人才项目资助(No.2007A卫01) （No.2007A卫01）
