南方医科大学学报Issue(9)：1547-1550,4.
早发型与晚发型重度子痫前期患者胎盘绒毛组织代谢足迹差异的研究
Metabolic footprint in conditioned culture medium of placental explants: a comparison between early-onset and late-onset severe preeclampsia
摘要
Abstract
Objective To explore the differences of metabolic footprint in the conditioned culture medium of placental explants between early-onset and late-onset severe preeclampsia. Methods In 13 cases of early-onset severe preedampsia and 14 cases of late-onset severe preeclampsia, the placentas were sampled at the surface of the maternal placenta. High performance liquid chromatography-mass spectrometry (HPLC-MS) was used to determine the differences in the metabolites in the conditioned culture medium of the placental villous explants cultured in 6% atmospheric O2 for 96 h. Standard samples were used to establish the tryptophan and kynurenine chromatography library by HPLC-MS to analyze the concentration of tryptophan and kynurenine in the conditioned culture medium. Results Thirty-six metabolites showed statistically significant differences between early-onset and late-onset severe preeclampsia (P<0.05). The concentration of kynurenine was significantly higher in early-onset severe preeclampsia than in late-onset severe preeclampsia (P<0.05). Conclusion Early-onset and late-onset severe preeclampsia may have different pathogeneses. By detecting the concentration of metabolites, metabolomic strategies provide a new means for predicting the onset time of severe preeclampsia.关键词
子痫前期/胎盘/代谢足迹/犬尿氨酸Key words
preeclampsia/ placenta/ metabolic footprint/ kynurenine分类
医药卫生引用本文复制引用
刘大艳,陈士岭,王晨虹,罗雪梅,黄芳芳..早发型与晚发型重度子痫前期患者胎盘绒毛组织代谢足迹差异的研究[J].南方医科大学学报,2011,(9):1547-1550,4.基金项目
深圳市科技计划项目(201003113) （201003113）
