中国中西医结合杂志Issue(10)：1401-1404,4.
丹酚酸B对转化生长因子β1诱导的人胚肺成纤维细胞生物学行为的影响
Effect of Salvianolic Acid B on TGF-β1-induced Human Embryonic Lung Fibroblast'S Biological Behavior
摘要
Abstract
Objective To investigate the effect of salvianolic acid B (SAB) on the proliferation of human embryonic lung fibroblast MRC-5, and the secretion of precollagenl and endogenous transforming growth factor-β1 (TGF-β1). Methods The MRC-5 cells were randomly divided into four groups as follows: the control group: cells cultured with DMEM but with no TGF-p, or SAB; the TGF-p, group: cell cultured with 10 ng/mL TGF-β1; the SAB1 group: cell cultured with medium with 10 ng/mL TGF-p, and 1 μmol/L SAB; the SAB2 group: cell cultured with medium with 10 ng/mL TGF-β1 and 10 μmol/L SAB. The proliferation of cells was assayed by MTT incorporation. The concentration of amino-terminal propeptide of type I procoflagen (PINP), a marker of collagen synthesis, was measured by radioimmunoassay. The endogenous TGF-β1 levels were measured using ELISA. Results The optical density, precollagenl contents, and endogenous TGF-β1 levels significantly increased when compared with those of the control group (P<0.05). Compared with the TGF-p, group, the optical density was obviously lowered, the precollagenl contents and endogenous TGF-β1 levels significantly decreased in the SAB1 group and the SAB2 group, and better in the SAB2 group, showing statistical difference (P<0.05). Conclusions SAB could inhibit the proliferation of MRC-5 cells induced by TGF-β1, and attenuate the roles of secreting collagen and endogenous TGF-β1. It had the potential of postponing or delaying the progressive developing of pulmonary fibrosis.关键词
丹酚酸B/肺成纤维细胞/增殖/转化生长因子β1/胶原Key words
salvianolic acid B/ pulmonary fibroblast/ proliferation/ transforming growth factor-β1/ collagen引用本文复制引用
张敏,冯志顺,邓哲彤,叶小玲,张锐,晋金兰,江颖仪..丹酚酸B对转化生长因子β1诱导的人胚肺成纤维细胞生物学行为的影响[J].中国中西医结合杂志,2011,(10):1401-1404,4.基金项目
广东省卫生厅资助项目(No.A2008537) （No.A2008537）
广州市中医药科研项目立项资助项目(2009) （2009）
