中国中西医结合杂志Issue(10)：1409-1413,5.
建立痛风性肾病并发慢性肾功能衰竭动物模型方法的探讨
Exploration on the Establishment of Animal Models for Gouty Nephropathy Complicated with Chronic Renal Failure
摘要
Abstract
Objective To explore the method for establishing animal models of gouty nephropathy complicated with chronic renal failure. Methods Six -eight weeks old male Wistar rats were fed with 10% fodder yeast. The adenine at the daily dose of 100, 150, 200, 250, and 300 mg/kg was administrated to them by gastrogav-age. The serum levels of blood urea nitrogen (BUN), creatinine (Cr), and uric acid (UA) were dynamically monitored. Meanwhile, the pathological changes of rat kidney were observed. Results Compared with the normal control group, serum BUN, Cr, and UA obviously increased in rats administered with 100 mg/kg for 7 days (P< 0. 05). Meanwhile, pathological changes as gouty nephropathy occurred. Along with the prolongation of the modeling time, the aforesaid biochemical indices and pathohistological changes of the kidney were more obvious. The blood Cr level just reached the chronic renal failure level on the 26th day of the administration (about the 4th week), and obviously exceeded the renal failure level on the 41 st day (about the 6th week). The blood UA level increased to a higher level on the 7th day of modeling, and maintained at a higher level for a long time. It decreased rapidly from the 41 st day to the 48th day. The renal pathological examination showed aggravated infiltration of lymphocytes and stromal fibrous proliferation. On the 48th day of modeling, the proliferation of the fibrious tissue and the interstitial fibrosis were obvious on the bases of the aforesaid changes. The serum BUN, Cr, and blood UA obviously increased in the rats administered with 150, 200, 250, and 300 mg/kg when compared with the normal control group, reaching the level of chronic renal failure (P<0. 05). These levels obviously decreased 17 days after restoring to normal fodder feeding, and approached the normal levels till the 35th day. Conclusion Ideal experimental animal models of gouty nephropathy complicated with chronic renal failure could be established in male Wistar rats by feeding with 10% fodder yeast and 100 mg/kg adenine by gastrogavage for 5 weeks.关键词
高尿酸血症/肾病/慢性肾功能衰竭/动物模型Key words
hyperuricemia/ nephropathy/ chronic renal failure/ animal model引用本文复制引用
邢儒伶,孟冬梅,任伟,李长贵..建立痛风性肾病并发慢性肾功能衰竭动物模型方法的探讨[J].中国中西医结合杂志,2011,(10):1409-1413,5.基金项目
山东省青岛市科技局资助项目(No.07-2-1-19-nsh) （No.07-2-1-19-nsh）
