南方医科大学学报Issue(9)：1605-1607,3.
FISH技术在快速诊断自然流产胚胎染色体数目异常中的应用
Rapid detection of chromosome number aberration in spontaneously aborted fetuses using fluorescence in situ hybridization
摘要
Abstract
Objective To assess the value of fluorescence in situ hybridization (FISH) in the diagnosis of common chromosome number aberration in spontaneously aborted fetuses. Method A total of 100 spontaneously aborted fetuses were analyzed by G-banding and by FISH to test chromosome number aberration mainly for chromosomes 13, 18, 21, X and Y, and the results of FISH test was assessed according to those by G-banding test. Results FISH results were well consistent with those by G-banding test. FISH test identified trisomy in 32 samples and polyploidy in 7 samples. Two samples with cell culture failure were found to have trisomy 16 by FISH. Discrepancies in the results between the two tests occurred in 3 samples, but the results of FISH were verified by other methods. Kappa test between FISH technology and G-banding showed a good consistency between FISH and karyotyping (P0.05). Conclusion FISH is an effective and rapid method for detecting chromosome number aberration in spontaneously aborted fetuses, and the combination of FISH and karyotyping provides more reliable diagnostic evidence.关键词
荧光原位杂交/染色体数目异常/自然流产Key words
fluorescence in situ hybridization/ chromosome number aberration/ spontaneous abortion分类
医药卫生引用本文复制引用
宋兰林,熊丽,刘思平,刘洁,钟梅,高云飞,吴瑞枫,余艳红..FISH技术在快速诊断自然流产胚胎染色体数目异常中的应用[J].南方医科大学学报,2011,(9):1605-1607,3.基金项目
中华医学会分子生物学临床应用研究专项资金(CAMB012010) （CAMB012010）
