土壤2011，Vol.43Issue(3)：433-438,6.
胡枝子高质量RNA的提取及ALMT基因片段的克隆和表达分析
Extraction of Total RNA and Cloning and Expression of ALMT Gene Fragment in Lespedeza bicolor
摘要
Abstract
The total RNA from the root and shoot of woody plant Lespedeza bicolor, which had plenty of polysaccharides and polyphenols were extracted by improved CTAB method. The values of OD260/OD280 of the extracted RNA were between 1.9-2.1, the yields were between 100-250 μg/g FW. ALMT gene fragment was successfully amplified from cDNAs reverse-transcribed from the total RNA and its expression was analyzed by semi-quantitative RT-PCR and quantitative real-time RT-PCR. The sequence of the cloned ALMT gene fragment in Lespedeza bicolor was highly identical to other species' ALMT gene sequences. This gene was Al-inducing and mainly expressed in roots. These results showed that the total RNA extracted from Lespedeza bicolor by this method was of high quality, and could be used for the following study on gene expression.关键词
胡枝子/改良CTAB法/RT-PCR/ALMT基因/铝Key words
Lespedeza bicolor, Improved CTAB method, RT-PCR, ALMT, Al分类
农业科技引用本文复制引用
陈志长,赵学强,沈仁芳..胡枝子高质量RNA的提取及ALMT基因片段的克隆和表达分析[J].土壤,2011,43(3):433-438,6.基金项目
国家自然科学基金项目(40871144,30821140538)和中国科学院南京土壤研究所知识创新工程领域前沿项目(ISSASIP0706)资助。 （40871144,30821140538）
