郑州大学学报（工学版）2011，Vol.32Issue(4)：99-102,4.
毛细管电泳-电致化学发光法测定盐酸林可霉素
Determination of Lincomycin Hydrochloride with Capillary Electrophoresis-Electrochemiluminescence
邵蓓新 1张颖 2李建军 2屈凌波2
作者信息
- 1. 郑州大学第五附属医院药剂科,河南郑州450052
- 2. 郑州大学化学系,河南郑州450001
- 折叠
摘要
Abstract
A method of capillary eclctrophoresis (CE) with tris(2,2'-bipyridyl) ruthenium ( II ) electro-chemiluminescence (ECL) detection has been developed for the determination of Lincomycin hydrochloride. The effects of detection potential, concentration and acidity of phosphate buffer, separation voltage, injection voltage of tris(2,2'-bipyridyl) and time,concentration and acidity of seperation buffer on the detection were investigated. Under optimized conditions, the calibration curve for Lincomycin hydrochloride showed a good linearity in the range of 5 x 10'8 ~9 x 10"' g/mL,and the detection limits was 1. 55 x 10~8 g/mL. The method was applied for the determination of lincomycin hydrochloride in the lincomycin hydrochloride injections with a recovery of 99. 62% ~ 100. 31 % .关键词
毛细管电泳(CE)/电致化学发光(ECL)/联吡啶钌(Ru(bpy)23+)/盐酸林可霉素Key words
capillary electrophoresis/electrochemiluminescence/tris (2,2' - bipyridyl) ruthenium ( II )/lincomycin hydrochloride分类
医药卫生引用本文复制引用
邵蓓新,张颖,李建军,屈凌波..毛细管电泳-电致化学发光法测定盐酸林可霉素[J].郑州大学学报（工学版）,2011,32(4):99-102,4.
