华西口腔医学杂志2011，Vol.29Issue(5)：546-549,4.DOI:10.3969/j.issn.1000-1182.2011.05.025
骨形态发生蛋白-2基因转染对舌癌细胞增殖活性的影响
Effects of bone morphogenetic protein-2 on proliferation of tongue cancer Tca8113
摘要
Abstract
Objective To investigate the morphology and proliferation effects of adenovirus containing bone morpho-genetic protein-2 (BMP-2) on tongue cancer Tca8113 cells. Methods Tca8113 cells were transfected with the Ad-BMP-2 of 0, 50, 100 multiplicity of infection (MOD respectively. Inverted fluorescence microscope was used to evaluate the morphological changes of these cells. Western blot analysis was performed to determine the expression levels of BMP-2 in the transfected cells. Methyl thiazolyl tetrazolium (MTT) assay was performed to monitor the proliferative activity of the infected Tca8113 cells and then the growth curve was made. Results The transfection efficiency reached the highest when the MOI was 100. Moreover, the expression of BMP-2 was detected in Tca8113 cells by Western blot. There were no obvious morphological changes of the Tca8113 cells before and after transfection. And the proliferation of transfected Tca8113 cells decreased compared with control. Conclusion Ad-BMP-2 gene can inhibit the proliferation of Tca8113 cells in vitro.关键词
骨形态发生蛋白-2/腺病毒/基因转染/舌癌细胞Key words
bone morphogenetic protein-2/ adenovirus/ gene transfection/ tongue cancer cells分类
医药卫生引用本文复制引用
姜娟,张君,王旭霞,薛立伟,宋洪宁,张茜..骨形态发生蛋白-2基因转染对舌癌细胞增殖活性的影响[J].华西口腔医学杂志,2011,29(5):546-549,4.基金项目
山东省科学技术发展计划基金资助项目(2010GSF10239) （2010GSF10239）
