中国组织工程研究与临床康复2011，Vol.15Issue(41)：7653-7656,4.DOI:10.3969/j.issn.1673-8225.2011.41.013
MG63和MG63/ADR骨肉瘤裸鼠模型血管内皮生长因子和核因子κB1的表达
Vascular endothelial growth factor and nuclear factor-kappa B1 expression in a nude mouse model of MG63 and MG63/ADR osteosarcoma
摘要
Abstract
BACKGROUND: Studies concerning sarcoma drug resistance found that vascular endothelial growth factor (VEGF) and nuclear factor-kappa B1 (NF-B1) may be closely related to drug resistance, but few studies are involved in osteosarcoma. Establishment of animal models and gene expression of VEGF and NF-B1 may reveal the association between genes and drug resistance in osteosarcoma. OBJECTIVE: To observe gene expression differences of VEGF and NF-B1 in a nude mouse model of MG63 and MG63/ADR osteosarcoma. METHODS: An adriamycin-resistant human osteosarcoma cell subline (MG63/ADR) was established in vitro using gradually increased concentration of adriamycin (ADM) in culture. BALB/C nude mice were divided into MG63 and MG63/ADR groups with 10 mice in each group. The animal model of osteosarcoma xenografts was constructed by subcutaneous inoculation of osteosarcoma MG63 and MG63/ADR cells respectively. Two group mice were killed at 6 weeks and gene expression differences of VEGF and NF-B1 in the tumor formation of two groups were detected using immunohistochemistry and RT-PCR method. RESULTS AND CONCLUSION: Both VEGF and NF-B1 expression in MG63/ADR group was significantly higher compared with the MG63 group. The upregulation of VEGF and NF-B1 may be an important reason of drug resistance in osteosarcoma, providing an experimental basis for exploring the molecular mechanism of drug resistance in osteosarcoma and finding more effective targets of osteosarcoma treatment.关键词
阿霉素/耐药/肿瘤/血管内皮生长因子/核因子κB1分类
基础医学引用本文复制引用
孙佼,张春林,吴小三,赵世昌,赵曜,曾炳芳..MG63和MG63/ADR骨肉瘤裸鼠模型血管内皮生长因子和核因子κB1的表达[J].中国组织工程研究与临床康复,2011,15(41):7653-7656,4.基金项目
国家自然科学基金资助项目(30700850). （30700850）
