中国组织工程研究与临床康复2011，Vol.15Issue(41)：7681-7684,4.DOI:10.3969/j.issn.1673-8225.2011.41.020
蛇床子素干预人增生性瘢痕成纤维细胞增殖及转化生长因子β1的表达
Effects of osthole on fibroblast proliferation and transforming growth factor beta 1 expression in hypertrophic scar tissue
侯晓华 1陈虹 2曹波2
作者信息
- 1. 武警医学院附属医院烧伤整形科,天津市,300162
- 2. 武警医学院生药学教研室,天津市300162
- 折叠
摘要
Abstract
BACKGROUND: Osthole exhibits inhibitory effects on human fibroblast proliferation and transforming growth factor beta 1 (TGF-1) expression in hypertrophic scar tissue, but the precise mechanisms remains poorly understood. OBJECTIVE: To investigate the effects of osthole on human fibroblast proliferation and TGF-1 expression in hypertrophic scar tissue. METHODS: Human hypertrophic scar fibroblasts cells were cultured in vitro and then treated by osthole at different concentrations. The growth inhibitory effects were observed by MTT assay and cell growth curve. The expression of TGF-1 was detected by immunohistochemistry. RESULTS AND CONCLUSION: Osthole could obviously inhibit the growth of human hypertrophic scar fibroblasts. MTT assay showed that osthole IC50 value toward hypertrophic scar fibroblasts was 15.2±2.0 μmol/L. Furthermore, the results of cell growth curve matched with the above results. Immunohistochemistry results showed that osthole could obviously inhibit TGF-1 expression in the fibroblasts derived from hypertrophic scar tissue compared with the control group (P < 0.05). These findings suggest that osthole strongly inhibits the growth of human hypertrophic scar fibroblasts and decreases the expression of TGF-1.关键词
蛇床子素/增生性瘢痕/成纤维细胞/细胞增殖/转化生长因子.1分类
医药卫生引用本文复制引用
侯晓华,陈虹,曹波..蛇床子素干预人增生性瘢痕成纤维细胞增殖及转化生长因子β1的表达[J].中国组织工程研究与临床康复,2011,15(41):7681-7684,4.
