中国组织工程研究与临床康复2011，Vol.15Issue(41)：7685-7688,4.DOI:10.3969/j.issn.1673-8225.2011.41.021
脑缺血再灌注模型大鼠跑台运动后海马神经再生和血管内皮生长因子mRNA 的变化
Effects of treadmill running on hippocampal neurogenesis and mRNA expression in vascular endothelial growth factor in a rat model of ischemia and reperfusion injury
杨若愚 1娄淑杰 2陈秀恩3
作者信息
- 1. 上海市黄浦区体育科学研究站,上海市,200010
- 2. 上海体育学院运动科学学院,上海市200438
- 3. 上海华东医院康复医学科,上海市200040
- 折叠
摘要
Abstract
BACKGROUND: Several studies have demonstrated that treadmill running can promote hippocampal neurogenesis in healthy rats. OBJECTIVE: To investigate the effects of treadmill running on hippocampal neurogenesis and mRNA expression in vascular endothelial growth factors (VEGF) in a rat model of ischemia and reperfusion injury. METHODS: The method of reversible middle cerebral artery occlusion (MCAO) was used to obtain rat models of ischemia and reperfusion injury. MCAO rat models were randomly divided into exercise group and control group. A sham-operated group was used. Rats in the control and sham-operated groups were fed without exercise. The exercise group rats were subjected to 7-day treadmill running. The exercise and control group rats were intraperitoneally injected with 5-bromodeoxyuridine (BrdU) solution. RESULTS AND CONCLUSION: Immunohistochemical staining results showed that BrdU-positive cells in the bilateral hippocampus and dentate gyrus were significantly more in the exercise group than in the control group (P < 0.01). Real time quantitative PCR results showed that mRNA expression in VEGF was significantly greater in the exercise group than in the control and sham-operated groups (P < 0.05). The results suggest that appropriate treadmill running can promote hippocampal neurogenesis and further enhance the mRNA expression in hippocampal VEGF in ischemia and reperfusion adult rats.关键词
跑台运动/缺血再灌注/海马/神经细胞/血管内皮生长因子/组织构建分类
医药卫生引用本文复制引用
杨若愚,娄淑杰,陈秀恩..脑缺血再灌注模型大鼠跑台运动后海马神经再生和血管内皮生长因子mRNA 的变化 [J].中国组织工程研究与临床康复,2011,15(41):7685-7688,4.
