中国中医药信息杂志2011,Vol.18Issue(10):55-58,4.DOI:10.3969/j.issn.1005-5304.2011.10.021
脑血宁注射液中栀子药材HPLC指纹图谱研究
Fingerprint of Gardeniajasminoides in Naoxuening Injection by HPLC
摘要
Abstract
Objective To establish the HPLC fingerprints of Gardenia jasminoides in Naoxuening injection. Methods The chromatographic seperation was performed on a phenomenex C18 column (4.6 mm X 250 mm, 5 μm) with a mobile phase consisting of methanol-water, gradient eluent, at the flow rate of 0.8 mL/min. The column temperature was 30℃, and the detection wavelength was 238 nm and 440 nm. Results The fingerprint of Gardenia jasminoides in Naoxuening injection was established, and fourteen common peaks in the chromatogram were marked with the parameter according to the technical requirements of the fingerprints of Chinese traditional medicine. The ratio of common peak areas was different in the fingerprints of Gardenia jasminoides from different places. Conclusion This method is accurate, reliable, and provides a basis for controlling the quality of Naoxuening injection.关键词
脑血宁注射液/栀子/高效液相色谱法/指纹图谱Key words
Naoxuening injection/Gardenia jasminoides: HPLC/fingerprint分类
医药卫生引用本文复制引用
王硕华,周晓东,唐骏,赵曦..脑血宁注射液中栀子药材HPLC指纹图谱研究[J].中国中医药信息杂志,2011,18(10):55-58,4.基金项目
珠海市科技局研究项目(PC20061018) (PC20061018)