山东农业科学Issue(7)：1-4,8,5.
利用cDNA-AFLP分析大白菜、萝卜及其杂交种差异表达基因
Analysis of Differentially Expressed Genes in Brassica rapa L.ssp.pekinensis, Raphanus sativus L.and Their Hybrid Using cDNA-AFLP
摘要
Abstract
For well understand the molecular mechanism of heterosis in Cruciferae vegetables, the differ entially expressed genes in Chinese cabbage ( Brassica rapa L. Ssp. Pekinensis ) Fushanbaotou, radish ( Rapha-nus sativus L. ) Tiaotouqing and their F1 hybrid were displayed by cDNA - AFLP. And 44 differential bands in F1 hybrid were obtained, which belonged to 40 unigenes. Among them, 43 bands could be found homologous sequences in NCBI/CenBank and only 32 bands,function had been known . According to sequence analysis, the function - known sequences participated in the following biological processes: carbon metabolism, hormone synthesis, transcription, cell cycle, cellular transport,signal transduction, stress response, and flower devel opment.关键词
基因差异表达/大白菜/萝卜/杂种F1/cDNA-AFLPKey words
Differential gene expression/ Brassica rapa L. Ssp. Pekinensis/ Raphanus sativus L./ Hybrid F1/ cDNA - AFLP分类
生物科学引用本文复制引用
张庶,李利斌,王凤德,刘立锋,李化银,高建伟,高玲..利用cDNA-AFLP分析大白菜、萝卜及其杂交种差异表达基因[J].山东农业科学,2011,(7):1-4,8,5.基金项目
国家“973”前期研究专项(编号:2009CB126002)、山东省现代农业产业技术体系——蔬菜体系项目和山东省优秀中青年科学家科研奖励基金(编号:BS2010SW027)资助. （编号:2009CB126002）
