山东农业科学Issue(7)：15-17,3.
转反义PlDγ基因小麦的品质性状分析
Analysis of Quality Characters for Transgenic Wheat with Anti-PLDγ Gene
摘要
Abstract
The antisense phospholipase Dγ (PLDγ) gene was transferred into the wheat variety LK906 by earstem injecting method. The quality characters were analyzed for transgenic plants by PCR and PCR - South-em techniques. The results of HMW - CS electrophoresis showed that the subunit composition of transgenic lines was altered. The receptor, s subunit 10 disappeared and the subunits 8 and 12 appeared in transgenic lines. The results of seed gliadin electrophoresis showed that there were significant differences between trans genic lines and receptor in the appearance of new bands and disappearance of the receptor, s, and the degree of expression was also different. All the quality characters were improved somewhat but the degree was differ ent among different transgenic lines.关键词
转基因小麦/品质分析/差异Key words
Transgenic wheat/ Quality analysis/ Difference分类
农业科技引用本文复制引用
王瑞霞,钱兆国,丛新军,牟秋焕,米勇,孙宪印,王超..转反义PlDγ基因小麦的品质性状分析[J].山东农业科学,2011,(7):15-17,3.基金项目
现代农业产业技术体系项目(nycytx-03)资助. （nycytx-03）
