中国肺癌杂志2011，Vol.14Issue(7)：561-567,7.DOI:10.3779/j.issn.1009-3419.2011.07.01
沉默血管生成素-2表达对人肺腺癌细胞生物学特性的影响
The Influence of Inhibiting Angiopoietin-2 on the Biological Characteristics of Bronchogenic Adenocarcinoma
摘要
Abstract
Background and objective It is well-known that angiopoietin-2 (Ang-2) plays an important role in the formation of the blood vascular system. Angiopoietin is involved in many diseases about angiogenesis such as tumor, so may have great prospects for the treatment of these diseases. The aim of this study is to evaluate the influence of inhibiting Ang-2 via adeno-associated virus induced RNA interference (RNAi) on the biological characteristics of bronchogenic adenocarcinoma. Methods AAV-Ang-2'Hrna driven by HI promoter was constructed to transfect A549 cell line. Normal and AAV-Null cell line were utilized in the control groups. The influence of RNAi on Ang-2 expression as well as the growth rate, tumorigenic efficiency, proliferation rate, apoptosis, and microvessel density of A549 cell line were analyzed. Results In vitro experiment indicated that the Ang-2 expression level (P<0.00l) and growth rate (P<0.001) of A549 cell line 48 h transfected with AAV-Ang-2'Hrna were significantly lower than those in the normal and AAV-Null cell lines. Cell cycle analysis showed the proliferation index (PI) of normal, AAV-Null, and AAV-Ang-2lhRNA transfected A549 cell line were 0.51±0.43, 0.48±0.29, and 0.26±0.31, respectively, which indicated the PI of AAV-Ang-2thRNA transfected cell line was significantly lower, compared with the normal and AAV-Null cell lines. In vivo experiment exhibited that AAV-Ang-2lhRNA transfected cell line possessed a lower mass and volumn of tumorrelative to two control groups. In addition, the apoptosis index (AI) of AAV-Ang-2lhRNA transfected, normal, and AAV-Null cell lines were (5.98±3.1l)%, (7.51±4.42)% and (17.O6±7.43)% respectively, which manifested that AAV-Ang-2"Hrna transfected cell line possessed a higher AI (P=0.005, P=0.007). A lower percentage of PCNA-positive cell was observed in AAV-Ang-2'hliNA transfected cell line (92.75±9.7)% as well, compared with the normal (85.8±11.8)96 and AAV-Null (69.8±16.5)% cell lines. Conclusion AAV-mediated expression of shRNA significantly reduces concentration of Ang-2 in AS49 cell line, lowers proliferation and growth rate and induce .apoptosis of A549 cell line.关键词
血管生成素-2；A549细胞株；腺相关病毒；RNA干扰Key words
Angiopoietin-2/A549 cell strain/Adeno-associated virus/RNA interference分类
医药卫生引用本文复制引用
李白翎,张冠鑫,侯霄雷,袁扬,刘晓红,龚德军,黄盛东..沉默血管生成素-2表达对人肺腺癌细胞生物学特性的影响[J].中国肺癌杂志,2011,14(7):561-567,7.基金项目
本研究受国家自然科学基金项目(No.30471718)和上海市科委重点基金项目(No.04JC14006)资助 （No.30471718）
