热带作物学报2011，Vol.32Issue(4)：632-639,8.
龙眼胚性愈伤组织miR398a前体的克隆及其在龙眼体胚发生过程中的表达分析
Cloning of miR398a Precursor Gene from Embryogenic Callus and Its Expression Analysis During Somatic Embryogenesis in Longan
摘要
Abstract
A miR398a precursor was cloned and its expression level was analyzed for elucidating the role of miR398a during longan somatic embryogenesis. The miR398a precursor was isolated by RT -PCR and its transcription levels were detected in longan embryogenic cultures at different developmental stages by real-time RT-PCR. The length of the miR398a precursor (GenBank loucs FJ973472)was 83 bp, including 21 bp mature sequence of miR398a. qPCR results indicated that the miR398a precursor was expressed in most of the tissues in longan embryogenic cultures at different developmental stages. The expression quantity of the miR398a precursor was incomplete compact pro-embryogenic cultures>globular embryos >compact pro-embryogenic cultures>embryogenic callus II >friable-embryogenic callus >Cotyledonary embryos >heart -shaped embryos >torpedo-shaped embryos. Longan miR398a precursor was highly conserved among different species and expressed with tissue specific and sequential characteristics during longan somatic embryogenesis.关键词
龙眼；体细胞胚胎发生；miR398a前体；克隆；表达Key words
Longan/Somatic embryogenesis/miR398a precursor/Cloning/Expression分类
农业科技引用本文复制引用
林玉玲,赖钟雄..龙眼胚性愈伤组织miR398a前体的克隆及其在龙眼体胚发生过程中的表达分析[J].热带作物学报,2011,32(4):632-639,8.基金项目
高等学校博士学科点专项科研基金项目(20093515110005)；国家自然科学基金项目“龙眼体胚发生过程中miRNA的分离与鉴定”(31078717)；国家科技支撑计划项目(2007BAD07B01). （20093515110005）
