热带作物学报2011，Vol.32Issue(4)：648-652,5.
利用DDRT-PCR分析茶树冬季冷驯化过程中基因表达的差异
Study on Diggerential Gene Expression of Tea Plant (Camellia sinensis) During Cold Acclimation in Winter
摘要
Abstract
Gene differential expression between three samples of tea plant (Camellia sinensis) during winter were preliminary study by mRNA differential display reverse transcription-PCR (DDRT-PCR). 31 differentially expressed cDNA fragments of steadily reamplification were obtained from the 134 fragments. 10 differentially expressed cDNA fragments were confirmed by the analysis of RT-PCR, which primers were according to the sequence of differentially expressed cDNA fragments. The length were between 200 bp and 300 bp. Among them, 6 were increasing and 4 were reducing. Through BLAST, five differentially expressed cDNA fragments were showed homology to nectarin HI (NEC3) mRA (3), glycerophosphoryl diester phosphodiesterase family protein mRNA (48), tubuLin-specific chaperone C-related mRNA (52), photosystem II protein Dl gene (80, 138), lecithin cholesterol acyltransferase family protein gene (147) respectively. The fragments of 15, 82 and 134 showed high homologous to hypothetical protein of Vitis vinifera. Might be novel genes. The fragment of 72 showed poor homologous to all known genes, might be novel genes.关键词
茶树；冷驯化；基因表达差异；DDRT-PCRKey words
Tea plant (Camellia sinensis)/Cold acclimation/Gene expression difference/mRNA differential display reverse transcription-PCR分类
农业科技引用本文复制引用
梅菊芬,汤茶琴,徐德良,王新超..利用DDRT-PCR分析茶树冬季冷驯化过程中基因表达的差异[J].热带作物学报,2011,32(4):648-652,5.基金项目
浙江省自然科学基金重点项目(Z3100473)；江苏省农业科技支撑计划(BE2008320-1)；现代农业(茶叶)产业技术体系项目(CARS-23). （Z3100473）
