湖北医药学院学报2011，Vol.30Issue(3)：258-262,5.
PEP-1-SOD1融合蛋白预处理对大鼠骨髓间充质干细胞在缺氧无血清条件下的保护作用
The Protective Effect of PEP-1-SOD1 Preconditioning Under Hypoxia and Serum Deprivation Condition in Rat Bone Marrow Mesenchymal Stem Cells
摘要
Abstract
Objective To investigate the ability of PEP-1-SOD1 fusion protein transducting into tat bone marrow mesenchy-mal stem cells ( MSCs) ,and its protective effects on MSCs under hypoxia and serum deprivation condition. Methods MSCs were cultured and characterized,the purified PEP-SOO1 and SOD1 was added in the third generation MSCs. The localization of PEP-1-SOD1 in MSCs was detected by immunofluorescecnce method,the PEP-1-SOD1 levels in MSCs were detected by semi-quantity Western blot. The activity of PEP-1-SOD1 in MSCs was detected by xanthine oxidase method. MSCs were pre-treated with 2.5ujnol/L PEP-1-SOD1 for43 minutes,and the cells were cultured under hypoxia and serum deprivation condition. The apoptosis of cells pretreated or untreated with PEP-1-SOD1 were analyzed by flow cytometry with annexin V FTFC/PI double staining after 24 hours. Results PEP-1-SOD1 fusion protein could be transduced into MSCs in tune and dose dependentand manner,distributed in cytoplasm and nuclei. The PEP-1-SOD1 preconditioning could significantly decreased the apoptosis inducing by hypoxia and serum deprivation (P <0.05). Conclusion PEP-1-SOD1 fusion protein could be efficiently transduced into MSCs,and protect the MSCs apoptosis from hypoxia and serum deprivation condition.关键词
PEP-1肽；骨髓间充质干细胞；Cu、Zn超氧化物歧化酶；缺氧Key words
PEP-1 peptide/Bone marrow mesenchymal stem cell/Cu,Zn-superoxide dismutase/ Hypoxia分类
医药卫生引用本文复制引用
董晓伟,王家宁,唐俊明,孔霞,郭凌郧,郑飞,张蕾,黄永章,杨建业..PEP-1-SOD1融合蛋白预处理对大鼠骨髓间充质干细胞在缺氧无血清条件下的保护作用[J].湖北医药学院学报,2011,30(3):258-262,5.基金项目
湖北省教育厅(T200811)；十堰市重大科技项目(2006030Z)。 （T200811）
